Tetraclinis articulata (Vahl) Mast. essential oil as a promising source of bioactive compounds with antimicrobial, antioxidant, anti-inflammatory and dermatoprotective properties: In vitro and in silico evidence.

Tetraclinis articulata is a known traditional medicinal plant used to manage various ailments, such as diabetes, rheumatism and infectious diseases. This study aims to determine the chemical constituents of T. articulata essential oil (EO) and to evaluate its in vitro antibacterial, anti-candidal, antioxidant, anti-inflammatory and dermatoprotective properties. In addition, a computational docking approach was used to predict the potential antioxidant, antibacterial, antifungal, anti-inflammatory, and cytotoxic properties of the identified compounds. The volatile oil obtained by hydrodistillation was characterized using gas chromatography-mass spectrometry (GC-MS). The antioxidant activity of T. articulata EO was investigated using three complementary assays: DPPH, ABTS and FRAP. Lipoxygenase (5-LOX) and tyrosinase enzymes were used to assess the anti-inflammatory and dermatoprotective effects of this oil. Moreover, disc-diffusion technique, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays were employed for the antimicrobial screening. The GC-MS analysis revealed that bornyl acetate (41.80 %), α-pinene (17.97 %) and camphor (15.97 %) are the major components of the studied EO. Moreover, T. articulata EO has exhibited promising antioxidant effect on FRAP, DPPH, and ABTS experiments. It also significantly inhibited 5-LOX (IC50 = 67.82 ± 0.03 µg/mL) and tyrosinase (IC50 = 211.93 ± 0.02 µg/mL). The results of MIC and MBC assays indicated that T. articulata EO is able to inhibit the growth of all tested bacteria (Gram + and Gram -) and Candida species. The ratio of tolerance level indicated that the tested oil was bactericidal against the Gram + bacteria and Candida species, whereas it has a bacteriostatic behavior against the Gram- bacteria. In light of these findings, T. articulata EO may be suggested as a potential pharmaceutical agent to prevent inflammation and skin problems and may serve as a natural antimicrobial and antioxidant alternative for sustainable application in food products.

Phenotypic, molecular, and symbiotic characterization of the rhizobial symbionts isolated from Acacia saligna grown in different regions in Morocco: a multivariate approach.

The introduced species Acacia saligna is a very promiscuous host as it can be efficiently nodulated with a wide range diversity of rhizobia taxa, including both fast and slow-growing strains. Fourteen nitrogen (N)-fixing bacteria were isolated from root nodules of wild Acacia saligna growing in distinct geographic locations in Morocco and were examined for their symbiotic efficiency and phenotypic properties. Multivariate tools, such as principal component analysis (PCA) and hierarchical clustering analysis (HCA), were used to study the correlation between phenotypic and symbiotic variables and discriminate and describe the similarities between different isolated bacteria with respect to all the phenotypic and symbiotic variables. Phenotypic characterization showed a variable response to extreme temperature, salinity and soil pH. At the plant level, the nodulation, nitrogen fixation, and the shoot and root dry weights were considered. The obtained results show that some of the tested isolates exhibit remarkable tolerances to the studied abiotic stresses while showing significant N2 fixation, indicating their usefulness as effective candidates for the inoculation of acacia trees. The PCA also allowed showing the isolates groups that present a similarity with evaluated phenotypic and symbiotic parameters. The genotypic identification of N2-fixing bacteria, carried out by the 16S rDNA approach, showed a variable genetic diversity among the 14 identified isolates, and their belonging to three different genera, namely Agrobacterium, Phyllobacterium and Rhizobium.

Combination of Chemically-Characterized Essential Oils from Eucalyptus polybractea, Ormenis mixta, and Lavandula burnatii: Optimization of a New Complete Antibacterial Formulation Using Simplex-Centroid Mixture Design.

This study aims to identify the volatile profile of three essential oils obtained from Eucalyptus polybractea cryptonifera (EPEO), Ormenis mixta (OMEO), and Lavandula burnatii briquet (LBEO) and to examine their combined antibacterial activity that affords the optimal inhibitory ability against S. aureus and E. coli using simplex-centroid mixture design and checkerboard assay. Essential oils (EOs) were isolated by hydrodistillation and characterized using gas chromatography-mass spectrometry (GC-MS) and gas chromatography coupled with flame-ionization detector (GC-FID). The antibacterial activity was performed using disc diffusion and microdilution assays. The chemical analysis revealed that 1,8-cineole (23.75%), p-cymene (22.47%), and α-pinene (11.20%) and p-menthane-1,8-diol (18.19%), α-pinene (10.81%), and D-germacrene (9.17%) were the main components detected in E. polybractea and O. mixta EOs, respectively. However, L. burnatii EO was mainly represented by linalool (24.40%) and linalyl acetate (18.68%). The EPEO, LBEO, and OMEO had a strong antibacterial effect on S. aureus with minimal inhibitory concentrations (MICs) values ranging from 0.25 to 0.5% (v/v). Furthermore, the combination of 1/2048 MICEPEO + 1/4 MICLBEO showed a synergistic antibacterial effect on S. aureus with a FIC index of 0.25, while the formulation of 1/4 MICEPEO + 1/4 MICOMEO demonstrated an antibacterial synergistic activity on E. coli with a FIC index of 0.5. Moreover, the simplex-centroid mixture design reported that the most effective combinations on E. coli and S. aureus correspond to 32%/28%/40% and 35%/30%/35% of E. polybractea, O. mixta, and L. burnatii, respectively. Presented information highlights the action of antibacterial formulations of these EOs and suggests their potential applications as alternatives to commercialized drugs to contract the development of bacteria causing serious infections and food deterioration.

Antioxidant, Volatile Compounds; Antimicrobial, Anti-Inflammatory, and Dermatoprotective Properties of Cedrus atlantica (Endl.) Manetti Ex Carriere Essential Oil: In Vitro and In Silico Investigations.

Cedrus atlantica (Endl.) Manetti ex Carriere is an endemic tree possessing valuable health benefits which has been widely used since time immemorial in international traditional pharmacopoeia. The aim of this exploratory investigation is to determine the volatile compounds of C. atlantica essential oils (CAEOs) and to examine their in vitro antimicrobial, antioxidant, anti-inflammatory, and dermatoprotective properties. In silico simulations, including molecular docking and pharmacokinetics absorption, distribution, metabolism, excretion, and toxicity (ADMET), and drug-likeness prediction were used to reveal the processes underlying in vitro biological properties. Gas chromatography-mass spectrophotometry (GC-MS) was used for the chemical screening of CAEO. The antioxidant activity of CAEO was investigated using four in vitro complementary techniques, including ABTS and DPPH radicals scavenging activity, ferric reductive power, and inhibition of lipid peroxidation (ß-carotene test). Lipoxygenase (5-LOX) inhibition and tyrosinase inhibitory assays were used for testing the anti-inflammatory and dermatoprotective properties. GC-MS analysis indicated that the main components of CAEO are ß-himachalene (28.99%), α-himachalene (14.43%), and longifolene (12.2%). An in vitro antimicrobial activity of CAEO was examined against eleven strains of Gram-positive bacteria (three strains), Gram-negative bacteria (four strains), and fungi (four strains). The results demonstrated high antibacterial and antifungal activity against ten of them (>15 mm zone of inhibition) using the disc-diffusion assay. The microdilution test showed that the lowest values of MIC and MBC were recorded with the Gram-positive bacteria in particular, which ranged from 0.0625 to 0.25 % v/v for MIC and from 0.5 to 0.125 % v/v for MBC. The MIC and MFC of the fungal strains ranged from 0.5 to 4.0% (MIC) and 0.5 to 8.0% v/v (MFC). According to the MBC/MIC and MFC/MIC ratios, CAEO has bactericidal and fungicidal activity. The results of the in vitro antioxidant assays revealed that CAEO possesses remarkable antioxidant activity. The inhibitory effects on 5-LOX and tyrosinase enzymes was also significant (p < 0.05). ADMET investigation suggests that the main compounds of CAEO possess favorable pharmacokinetic properties. These findings provide scientific validation of the traditional uses of this plant and suggest its potential application as natural drugs.

Phytochemical analysis and evaluation of antimicrobial, antioxidant, and antidiabetic activities of essential oils from Moroccan medicinal plants: Mentha suaveolens, Lavandula stoechas, and Ammi visnaga.

Mentha suaveolens, Lavandula stoechas, and Ammi visnaga are widely used in Moroccan folk medicine against several pathological disorders, including diabetes and infectious diseases. This work was designed to determine the chemical profile of M. suaveolens (MSEO), L. stoechas (LSEO), and A. visnaga (AVEO) essential oils and assess their antimicrobial, antioxidant, and antidiabetic effects. The volatile components of LSEO, AVEO, and MSEO were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The in vitro antidiabetic activity was assessed using α-amylase and α-glucosidase enzymes, while DPPH, FRAP, and ß-carotene/linoleic acid methods were used to determine the antioxidant capacity. The antimicrobial activities were investigated using disc diffusion and broth-microdilution assays. GC-MS investigation revealed that the main components were fenchone (29.77 %) and camphor (24.9 %) for LSEO, and linalool (38.29 %) for AVEO, while MSEO was mainly represented by piperitenone oxide (74.55 %). The results of the antimicrobial evaluation showed that all examined essential oils (EOs) had noticeable antimicrobial activity against both bacteria and yeast, especially Micrococcus luteus and Bacillus subtilis. The MIC, MBC, and MFC values were ranged from 0.015 % to 0.5 %. The MBC/MIC and MFC/MIC ratios were less than or equal to 4.0 % (v/v), indicating their noticeable bactericidal and candidacidal efficacy. Moreover, the three EOs showed significant inhibitory effects on α-amylase and α-glucosidase (p < 0.05). It also exerted remarkable activity on FRAP, ß-carotene, and DPPH radicals. These findings demonstrated that the tested plants have promising biological activities, validating their ethnomedicinal value and providing potential applications as natural drugs.

Exploring Antimicrobial Features for New Imidazo[4,5-b]pyridine Derivatives Based on Experimental and Theoretical Study.

5-bromopyridine-2,3-diamine reacted with benzaldehyde to afford the corresponding 6-Bromo-2-phenyl-3H-imidazo[4,5-b]pyridine (1). The reaction of the latter compound (1) with a series of halogenated derivatives under conditions of phase transfer catalysis solid-liquid (CTP) allows the isolation of the expected regioisomers compounds (2-8). The alkylation reaction of (1) gives, each time, two regioisomers, N3 and N4; in the case of ethyl bromoactate, the reaction gives, at the same time, the three N1, N3 and N4 regioisomers. The structures of synthesized compounds were elucidated on the basis of different spectral data (1H NMR, 13C NMR), X-Ray diffraction and theoretical study using the DFT method, and confirmed for each compound. Hirshfeld surface analysis was used to determine the intermolecular interactions responsible for the stabilization of the molecule. Density functional theory was used to optimize the compounds, and the HOMO-LUMO energy gap was calculated, which was used to examine the inter/intra molecular charge transfer. The molecular electrostatic potential map was calculated to investigate the reactive sites that were present in the molecule. In order to determine the potential mode of interactions with DHFR active sites, the three N1, N3 and N4 regioisomers were further subjected to molecular docking study. The results confirmed that these analogs adopted numerous important interactions, with the amino acid of the enzyme being targeted. Thus, the most docking efficient molecules, 2 and 4, were tested in vitro for their antibacterial activity against Gram-positive bacteria (Bacillus cereus) and Gram-negative bacteria (Escherichia coli). Gram-positive bacteria were more sensitive to the action of these compounds compared to the Gram-negative, which were much more resistant.

Simplex-centroid design as innovative approach in the optimization of antimicrobial effect of Thymus satureioides, Myrtus communis and Artemisia herba alba essential oils against Escherichia coli, Staphylococcus aureus and Candidatropicalis.

Essential Oils (EOs) known since Antiquity, and initially obtained by maceration of aromatic plants, are used as diseases' remedies because they contain valuable therapeutic components. Antimicrobial effect's evaluation of formulations established by an augmented centered mixing plan for three Moroccan medicinal and aromatic plants' essential oils (EOs): Thymus satureioides (T. satureioides), Myrtus communis (M. communis) and Artemisia herba alba (A. herba alba) against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Candida tropicalis (C. tropicalis). The identification of E.Os chemical compounds was made using Gaz chromatography (GC) and Gaz chromatography coupled to Mass spectrometry (GC/MS).Whereas, the optimization of their antimicrobial effect was performed by experimental design methodology (mixture design) coupled to microdilution method based on Minimal Inhibitory Concentrations (MICs) determination. Results showed that the main components are borneol (34.26%) and α-pinene (31.21%); borneol (27.15%) and 1,8-cineole (21.33%); camphor (14.67%), chrysanthenone (14.10%), and ß-thujone (12.60%) for T. satureioides, M. communis and A. herba alba respectively. Optimal mixtures composed of 60% and 40% of T. satureioides and M. communis E.Os; 72% and 28% of T. satureioides and A. Herba alba E.Os; 75% of thyme E.O and 25% of mugwort E.O; predicted the highest antimicrobial effect against E. coli, S. aureus and C. tropicalis, respectively. This study highlights the antimicrobial effect of EOs tertiary mixtures through using mixture design methodology, which may contribute to a successful application in pharmaceutical, food, or cosmetic industries.

Moroccan Medicinal Plants Used to Treat Cancer: Ethnomedicinal Study and Insights into Pharmacological Evidence.

Cancer is one of the major medical challenges, with an unacceptably high death toll worldwide. In Morocco, medicinal plants continue to play a pivotal therapeutic role despite the development of modern sanitation systems. In the current study, an ethnobotanical survey was carried out at the Moroccan national institute of oncology, Rabat, and we aimed at (1) establishing an exhaustive inventory of indigenous knowledge of Moroccan medicinal plants used to manage cancer and (2) confirming the reported ethnopharmacological uses through bibliometric review. An ethnobotanical survey was conducted with 291 cancer patients at the Moroccan National Institute of Oncology, Rabat, during a period of 4 months, from February to May 2019, through semistructured interviews. Ethnobotanical indices, including informant consensus factor (FIC), use report (UR), relative frequency citation (RFC), botanical family use value (FUV), fidelity level (FL), and index of agreement on remedies (IAR), were employed in data analyses. The survey revealed that 39 medicinal plants belonging to 27 botanical families and 38 genera were used to treat cancer. The most used ethnospecies were Aristolochia longa with the highest RFC value (0.096), followed by Nigella sativa, Ephedra alata, Euphorbia resinifera, and Lavandula dentata, éwith RFC values of 0.072, 0.054, 0.044, and 0.044, respectively. In regard to the plant families, Lamiaceae contributed the highest number of plants with five species (FUV = 0.034), followed by Asteraceae (4 species; FUV = 0.020), and Fabaceae (4 species; FUV = 0.020). The leaves are the most popular plant part used by the studied population against cancer; otherwise, decoction was the most commonly used method for remedy preparation and the highest FIC was noticed for uterine cancer treatment (0.86). Considering these findings, further investigations into the recorded plant species should be performed to assess phytochemical constituents and pharmaceutical benefits in order to identify their active compounds for any drug formulations.

Incidence of surgical site infections and prediction of risk factors in a hospital center in Morocco.

INTRODUCTION: Surgical site infections (SSIs) remain the major cause of morbidity and mortality in the postoperative period and are important surgical and hospital quality indicators. In this context, our study aims to identify SSIs associated risk factors and to develop a predictive model. METHODOLOGY: 2521 patients who underwent surgery, between June 2018 and May 2019, in four surgery departments, at the Taza Provincial Hospital (Morocco) were diagnosed for SSI according to the standards of the Center for Disease Control and Prevention. The SSIs' risk factors were assessed by univariate statistical analysis and logistic regression using the Scikit Learn function of Python. RESULTS: The average age of the studied population was 35 ± 1 years. The overall SSI incidence was 6.3% (17.95%, 6.86%, 6.67% and 3.16% respectively in child, female, male and gynaecological-obstetrical surgeries. The univariate statistical analysis has shown a highly significant (p < 0.001) and a very significant (p < 0.01) relationship between SSIs and almost all risk factors; and the logistic regression model has revealed a strong association between SSI and people who have had previous surgery, urinary catheter, antibiotic use duration, co-morbidity, American Society of Anesthesiologists (ASA) score, duration of intervention, emergency preoperative and postoperative durations, service, specialty and age range. The prediction score exceeds 96% which justifies our model's quality. CONCLUSIONS: SSIs are generally frequent among postoperative patients. Therefore, pre-operative preparation, post-operative surveillance and the environment quality of the wards are necessary to reduce SSI rates in the hospital.

Characterization of Ziziphus lotus' Activated Carbon and Evaluation of Its Adsorption Potential.

This study aims to prepare activated carbon from an interesting biomaterial, corresponding to the cores of Ziziphus lotus, for the first time to the best of our knowledge, according to a manufacturing process based on its chemical and thermal activation. These cores were chemically activated by sulfuric acid for 24 h and then carbonized at 500°C for 2 hours. The obtained activated carbon was characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and Brunauer-Emmett-Teller (BET) analysis. The adsorption of methylene blue (MB) on the activated carbon was evaluated, by Langmuir and Freundlich models examination, in order to explain the adsorption efficiency in a systematic and scientific way. Moreover, pseudo-first-order and pseudo-second-order kinetic models were used to identify the mechanisms of this adsorption process. The characterization results showed an important porosity (pore sizes ranging from 10 to 45 µm), a surface structure having acid groups and carboxylic functions, and a specific surface of 749.6 m2/g. Results of the MB adsorption showed that this process is very fast as more than 80% of MB is adsorbed during the first 20 minutes. In addition, increasing the contact time and temperature improves the MB removal process efficiency. Moreover, this adsorption's kinetic modeling follows the pseudo-second-order model. Furthermore, data on the adsorption isotherm showed a maximum adsorption capacity of 14.493 mg/g and fit better with the Langmuir model. The thermodynamic parameters (∆G0, ∆S0, and ∆H0) indicate that the adsorption process is endothermic and spontaneous. Therefore, Ziziphus lotus can be used as a low-cost available material to prepare a high-quality activated carbon having a promising potential in the wastewater treatment.

The Relationship between Nasal Carriage of Staphylococcus aureus and Surgical Site Infections in a Hospital Center in Morocco.

BACKGROUND: Surgical site infection is a major public health problem in the world. Nasal carriage is a major risk factor for the development of nosocomial Staphylococcus aureus infection, especially methicillin-resistant Staphylococcus aureus (MRSA). Our work aims to determine the prevalence of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and the associated risk factors and to evaluate their sensitivity to 27 antibiotics. METHODS: A cross-sectional study was carried out on 100 patients, hospitalized in preoperative care of surgery units at the Taza Provincial Hospital Center in the Fez-Meknes region, from January to June 2019. Samples were taken from the patient's anterior nostril using single-use sterile dry or wet cotton swabs and then analyzed in the Provincial Public Health Laboratory in Taza. The carriage of Staphylococcus aureus was studied by conventional bacteriological methods by spreading nasal swabs on Chapman culture medium, while antibiotic resistance was determined by the Mueller-Hinton agar disc diffusion method according to the recommendations described by the Antibiogram Committee of the French Society of Microbiology 2019 (CA SFM 2019). RESULTS: Of the 84 patients found to be positive, 45.24% had coagulase-positive Staphylococcus aureus and 54.76% had coagulase-negative Staphylococcus. After surgery in the postoperative phase, 16 patients developed surgical site infections, of which two had a negative nasal culture and 14 had positive nasal culture. Among the Staphylococcus aureus-positive patients, 36.84% were colonized by a methicillin-resistant Staphylococcus aureus (MRSA) and 63.16% by a methicillin-sensitive Staphylococcus aureus (MSSA). Of these, 57.14% of MRSA colonized patients developed an infection of the surgical site and 42.85% showed no sign of SSI, while for patients colonized by MSSA, 16.67% developed SSI and 83.33% showed no sign of SSI. Moreover, children were the most affected by MRSA. Concerning antibiotic sensitivity, multiresistance of MRSA to more than 3 antibiotics has been found. CONCLUSION: To the best of our knowledge, this is the first study carried out in this hospital center with the aim of knowing the prevalence of nasal carriage of Staphylococcus aureus and MRSA and to identify the risk factors in order to prevent infections related to nasal carriage of Staphylococcus aureus and MRSA.

Main Therapeutic Uses of Some Moroccan Hot Springs' Waters.

Morocco has an important groundwater reserve, especially in the Atlas domain, corresponding to its largest water reservoir. This reserve comes from rainwater infiltrated into rocks and sediments to give rise to mineralized waters feeding many springs and having curative properties, which confer each spa-specific therapeutic indications, based on the medicinal properties of its waters. All over the world, mineral waters of thermal springs have interesting therapeutic uses to cure some diseases; unfortunately, such potential is underexploited in Morocco. This narrative review deals with the distribution of thermal springs in Morocco, the classification of their thermal waters, and their health effects, with the aim to enhance them. For this purpose, previous studies' results on different aspects of thermal waters were searched in the most famous scientific databases, by using targeted specific keywords. Literature has shown that Morocco contains several thermal springs, scattered throughout the Middle Atlas, in the South, and in the Rif, which are regarded as waters of high therapeutic quality, whose thermal cures are often recommended to people suffering from rheumatism and locomotor system's diseases, skin diseases such as eczema and psoriasis, cardiovascular diseases, overweight, or respiratory troubles. However, apart from the unique and real thermal station of Moulay Yacoub, the other thermal springs are exploited in an empirical and artisanal way, mainly by a local population. So, as hydrotherapy constitutes a good choice of natural therapy using water components as a complement or alternative to conventional treatments, a better understanding of the thermal springs' distribution in Morocco, their thermal waters' classification, and their potential health effects may enable their valorization through a better use of their waters.

Microbiological Monitoring of the Environment Using the "Association Rules" Approach and Disinfection Procedure Evaluation in a Hospital Center in Morocco.

Background: The hospital environment, especially surfaces and medical devices, is a source of contamination for patients. Objective: This study carried out, to the best of our knowledge, for the first time at Taza Hospital in Morocco aimed to assess the microbiological quality of surfaces and medical devices in surgical departments and to evaluate the disinfection procedure in time and space. Methods: Samples were taken by swabbing after cleaning the hospital surface or medical device, to isolate and identify germs which were inoculated on semiselective culture media then identified by standard biochemical and physiological tests, using the analytical profile index (API) galleries. Moreover, the association rules extraction model between sites on the one hand and germs on the other hand was used for sampling. Results: The study showed that 83% of the samples have been contaminated after biocleaning. The most contaminated services have been men's and women's surgeries. 62% of isolated germs have been identified as Gram-positive bacteria, 29% as Gram-negative bacteria, and 9% as fungi. Concerning the association rules extraction model, a strong association between some contaminated sites and the presence of germ has been found, such as the association between wall and nightstand and door cuff, meaning that the wall and nightstand contamination is systematically linked to that of the door cuff. The disinfection procedure efficacy evaluation has enabled suggesting renewing it each 4 h. Conclusion: Microbiological monitoring of surfaces is necessary at hospital level through the use of the association rule extraction model, which is very important to optimize the sampling, cleaning, and disinfection site scenarios of the most contaminated ones.

Ethnopharmacology, Phytochemistry, and Pharmacological Properties of Thymus satureioides Coss.

Thymus satureioides Coss. (Lamiaceae) is a Moroccan medicinal plant locally known as "Azkouni" or "Zaitra." It is widely used in traditional medicine to treat various ailments, including hypertension, diabetes, cold, fever, dermatological and circulatory disorders, immune problems, bronchitis, nociception, cooling, pharyngitis, cough, and influenza. The current review aims to critically summarize the literature on ethnopharmacological uses, chemical profile, and pharmacological investigations of T. satureioides in order to provide data support and scientific evidences for further investigations. Electronic databases such as Scopus, PubMed, Web of Science, SciFinder, ScienceDirect, Google Scholar, and Medline were used to gather data on T. satureioides. Chemical characterization of T. satureioides essential oils (EOs) and extracts allowed to identify a total of 139 bioactive compounds, mainly belonging to the terpenoids, phenolic acids, and flavonoids classes. T. satureioides especially its essential oils exhibited numerous biological activities such as antibacterial, antifungal, anti-inflammatory, antioxidant, antidiabetic, anticancer, antiparasitic, and hypolipedemic activities. In light of these findings, further studies to transmute the traditional application of T. satureioides into scientific-based information are strongly required. Additional in vivo pharmacological studies are recommended to validate the results of the in vitro studies. Moreover, comprehensive preclinical and clinical trials on the pharmacological mechanisms of action of this plant and its bioactive compounds on molecular targets should be performed. Finally, more efforts must be focused on toxicological assessments and pharmacokinetic studies, in order to ensure the safety and the efficiency of T. satureioides.

Environmental Impact Assessment of Taza City's Wastewater: Application through Principal Component Analysis.

Taza City is among the Moroccan cities which is in full urban expansion, with a daily wastewater discharge volume estimated at 16534 m3/d in 2020, and expected to reach 20056 m3/d by 2030. These waters, collected in a combined sewerage network, are directly released into the natural environment without any treatment. Indeed, a large part of this water is discharged into Oued Defali, the main tributary of Oued Larbâa. In order to manage and better understand these discharges impact on the streams crossing this city, wastewater sampling campaigns were carried out for one year from May 2018 to April 2019 at domestic (S1) and industrial (S2) sites. The wastewater physicochemical characterization revealed that these discharges are highly loaded with organic matter in terms of chemical oxygen demand (S1 avg = 1231.44 mg/l and S2 avg = 933.03 mg/l), biochemical oxygen demand (S1 avg = 511.87 mg/l and S2 avg = 464.35 mg/l), and suspended matter (S1 avg = 744.11 mg/l and S2 avg = 578.13 mg/l). The use of principal component analysis (PCA) has allowed us to collect as much information as possible from the database of the physicochemical analyses performed for the studied parameters.

Screening and optimization of indole-3-acetic acid production and phosphate solubilization by rhizobacterial strains isolated from Acacia cyanophylla root nodules and their effects on its plant growth.

BACKGROUND: Plant growth-promoting rhizobacteria (PGPR) are known to improve plant growth and are used as biofertilizers, thanks to their numerous benefits to agriculture such as phosphorus solubilization and phytohormone production. In this paper, four rhizospheric bacteria (Phyllobacterium sp., Bacillus sp., Agrobacterium sp., and Rhizobium sp.) isolated from surface-sterilized root nodules of Acacia cyanophylla were tested for their ability to solubilize inorganic phosphate and to produce indole-3-acetic acid (IAA) under laboratory conditions. Then, the best IAA producer (Rhizobium sp.) was selected to test optimized conditions for IAA production. Finally, the effect of the four strains on plant growth for A. cyanophylla was evaluated in vivo. RESULTS: The results showed that the totality of the tested isolates had solubilized inorganic phosphate (P) in both NBRIP (National Botanical Research Institute Phosphate) and PVK (Pikovskaya) media. Bacillus sp. was a high P-solubilizer and showed maximum solubilization in PVK (519 µg ml-1) and NBRIP (782 µg ml-1). The optimization of maximum phosphate solubilization was done using different sources of carbon (1%) and nitrogen (0.1%). Glucose and ammonium sulfate were selected to be the best carbon and nitrogen source for phosphate solubilization by all tested strains, except for Phyllobacterium sp., which recorded the highest phosphate solubilization with ammonium nitrate. The IAA production by the tested strains indicated that Rhizobium sp. produced the highest amount of IAA (90.21 µg ml-1) in culture media supplemented with L-tryptophan. The best production was observed with L-Trp concentration of 0.2% (116.42 µg ml-1) and at an initial pH of 9 (116.07 µg ml-1). The effect of NaCl on IAA production was tested at concentrations of 0 to 5% and the maximum production of 89.43 µg ml-1 was found at 2% NaCl. The extraction of crude IAA from this strain was done and purity was confirmed with Thin Layer Chromatography (TLC) analysis. A specific spot from the extracted IAA production was found to correspond with a standard spot of IAA with the same Rf value. Finally, the tested PGPR demonstrated growth stimulatory effects on Acacia cyanophylla seedlings in vivo, with a great increase of shoots' and roots' dry weights, and shoot length compared to control. The rhizobacterial isolates were identified by 16S rDNA sequence analysis as Agrobacterium sp. NA11001, Phyllobacterium sp. C65, Bacillus sp. CS14, and Rhizobium sp. V3E1. CONCLUSION: This study highlights the importance of the use of phosphate solubilizing and IAA producer microorganisms as biofertilizers to increase crop yields. The studied strains showed a significant phosphate solubilization potential and IAA production. The use of selected strains as inoculants would be interesting, in particular with a view of promoting sustainable agriculture. However, further studies to verify the efficacy of the best isolates in situ is certainly required.

Screening and optimization of indole-3-acetic acid production by Rhizobium sp. strain using response surface methodology.

BACKGROUND: The production of indole-3-acetic acid (IAA) is an essential tool for rhizobacteria to stimulate and facilitate plant growth. For this, eighty rhizobial bacteria isolated from root nodules of Acacia cyanophylla grown in different regions of Morocco were firstly screened for their ability to produce IAA. Then, IAA production by a combination of isolates and the inoculation effect on the germination of Acacia cyanophylla seeds was studied using the best performing isolates in terms of IAA production. The best IAA producer bacterial isolate (I69) was selected to optimize IAA production using response surface methodology based on the central composite design. RESULTS: Results showed that the majority of tested isolates were able to produce IAA with a relatively higher concentration of 135 µg/ml for the isolate I69, followed by isolates I22 and I75 with respective concentrations of 116 µg/ml and 105 µg/ml IAA. The IAA production and the seed germination rate were relatively increased by the synergistic effect of I69 and I22. Later, response surface methodology was used to determine optimal operating conditions leading to IAA production optimization. Thus, an incubation temperature of 36 °C, a pH of 6.5, an incubation time of 1 day, and respective tryptophan and NaCl concentrations of 1 g/l and 0.1 g/l were optimal parameters leading to 166 µg/ml IAA which was the maximal produced concentration. CONCLUSION: The present study highlighted that IAA-producing rhizobacteria could be harnessed to improve plant growth. Furthermore, their production can be easily controlled using response surface methodology, which represents a very useful tool for optimization.

Characterization, antioxidant, antimycobacterial, antimicrobial effcts of Moroccan rosemary essential oil, and its synergistic antimicrobial potential with carvacrol.

Thanks to their promising properties, essential oils (EOs) have strong potential to remedy several problems such as microorganisms acquired resistance to antimicrobial agents and chemical antioxidants toxicity. Firstly, this work was conducted to determine chemical composition, antioxydant activity, then antibacterial, antifungal, antimycobacterial properties of Rosmarinus officinalis EO. Secondly, EOs combined antimicrobial effect with carvacrol was assessed. Chemical EO analysis was performed using Gas chromatography/mass spectrometer. 1,1-diphenyl-2-picrylhydrazyl test was used to evaluate in vitro antioxidant rosemary oil effect. The antimicrobial activity against seven bacteria, two fungi, and two mycobacterial strains was screened using the broth microdilution method. Thereafter, the checkerboard essay was used to evaluate the antibacterial effect of this EO and Carvacrol. Chemical EO analysis revealed 1,8-cineole (33.88%), camphor (14.66%), and α-pinene (12.76%) as main constituents. The obtained IC50 value (2.77 mg/mL) showed rosemary EO's radical scavenging power. Moreover, all tested microorganisms showed an important sensitivity to this EO (MIC values: 0.007%-1% (v/v)). Furthermore, results highlighted synergistic and partial synergistic interaction for tested products. The studied EO has both antimicrobial and antioxidant potentials. Combined application showed a remarkable synergistic antibacterial potentiation that can be used as an alternative in pharmaceutical and food processing sectors.

Antibacterial, antifungal and antioxidant activity of total polyphenols of Withania frutescens.L.

Our objective in this work is evaluated the antibacterial, antifungal and antioxidant activity of the phytochymic compounds of the roots and leaves of a species Withania frutescens. In the first part, the phenolic compound is determinate by the Folin-Ciocalteau reaction, the richness of the roots in polyphenols (53.33 ±â€¯1.20 mg EGA/g Extract) is six times higher than that of the leaves. The antioxidant test is evaluated by four methods: DPPH test, reducing power test (FRAP), total antioxidant capacity (CAT) and the ß-carotene discoloration test. The IC-50 values of the DPPH test of the studied parts are of the order of 0.36 µg/ml and 6.63 µg/ml, which showed a lower anti-free radical activity than that of BHT (0.12 µg/ml). The results obtained by the FRAP method revealed a low reducing power of iron for two extracts (EC-50 of 0.45%) compared to Quercetine (EC-50 of 0.03%). The compounds of root and leaf extracts have a significant total antioxidant capacity, respectively 477.65 ±â€¯37.60 and 317.03 ±â€¯46.64 mg EAA/g Extract. In the ß-carotene discoloration test, extracts from the aerial and underground parts showed antioxidant activity of 57% followed by (36%), respectively. The evaluation of the antibacterial activity of in vitro extracts against microorganisms is carried out by two methods: disc diffusion and microdilution. The results show that the extracts exert an intermediate inhibitory effect (inhibition diameter between 8 and 15 mm, the smallest MIC obtained is 2.80 mg/ml) on all strains tested. The antifungal activity was estimated by determining the growth inhibition rate of the fungus tested. Indeed, the compounds studied exhibit a good antifungal effect since the minimum inhibitory concentration (MIC) of 4.5 mg/ml for root extract and 9 mg/ml for leaf extract.

Combined treatment of Thymus vulgaris L., Rosmarinus officinalis L. and Myrtus communis L. essential oils against Salmonella typhimurium: Optimization of antibacterial activity by mixture design methodology.

To increase the sensibility of Salmonella typhimurium strain, a mixture of Thymus vulgaris L. (T. vulgaris L.), Rosmarinus officinalis L. (R. officinalis L.) and Myrtus communis L. (M. communis L.) essential oils (EOs) was used in combined treatment by experimental design methodology (mixture design). The chemical composition of EOs was firstly identified by GC and GC/MS and their antibacterial activity was evaluated. The results of this first step have shown that thymol and borneol were the major compounds in T. vulgaris and M. communis L. EOs, respectively, while 1,8-cineole and α-pinene were found as major compounds in R. officinalis L. The same results have shown a strong antibacterial activity of T. vulgaris L. EO followed by an important power of M. communis L. EO against a moderate activity of R. officinalis L. EO. Besides, 1/20 (v/v) was the concentration giving a strain response classified as sensitive. From this concentration, the mixture design was performed and analyzed. The optimization of mixtures antibacterial activities has highlighted the synergistic effect between T. vulgaris L. and M. communis L. essential oils. A formulation comprising 55% of T. vulgaris L. and 45% of M. communis L. essential oils, respectively, can be considered for the increase of Salmonella typhimurium sensibility.